High-Resolution Analysis of Intact Triglycerides by Reversed Phase HPLC Using the Agilent 1290 Infinity LC UHPLC System
نویسنده
چکیده
The Agilent 1290 Infinity LC System with ultraviolet/visible (UV/VIS) Diode Array detection (DAD) is used to analyze triglycerides in soybean oil under non-aqueous reversed phase gradient conditions. The Agilent 1290 Infinity LC System was used for the chromatographic separation of the sample on 3.0 and 2.1 mm id C18 columns, of various lengths, with 1.8-μm packing materials prepared in 600 bar (9000 psi) or special 1200 bar (18,000 psi) configurations. The ability of the Agilent 1290 Infinity LC System to operate with long, high resolution columns is demonstrated with isopropanol (IPA) or methyl tert butyl ether (MTBE) as the strong solvent and acetonitrile as the weak component of the mobile phase mixture. Authors
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